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Extracellular Anti-melanogenic from plants has been investigated extensively as a natural alternative to chemical-based therapies, free of negative effects. Extracellular vesicles (EVs), which were taken from the leaves and stems of S. japonica, were the subject of recent research that looked at their ability to inhibit melanogenesis. We found that both leaf-derived extracellular vesicles (LEVs) and stem-derived extracellular vesicles (SEVs) significantly decreased melanin content and Tyrosinase (TYR) activity in the B16BL6 murine melanoma cell line using spectrophotometric and biochemical methods.
Many tests and studies proved that both LEVs and SEVs reduced the amount of melanin in melanoma cells in a concentration-dependent manner. Both LEVs and SEVs demonstrated a superior whitening impact on melanoma cells when compared to arbutin, a frequently used positive control, with LEVs being particularly effective.
Notably, neither LEVs nor SEVs showed any discernible cytotoxicity. We also looked into how tyrosinase-related proteins (TRPs) are expressed in melanoma cells when extracellular vesicles from plants are present. The expression of melanogenesis-related genes and proteins, such as TYR, TRP-1, and TRP-2, as well as the microphthalmia-related transcription factor (MITF), was significantly suppressed by LEVs. In comparison to arbutin, LEVs showed a more effective inhibitory effect on melanin synthesis in a human epidermal model.
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